Primer express software 3.0

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Take the FileFixation tour now for more detailed information! Sequence Quality. Upload the settings from a file. All rights reserved. Steve Rozen and Helen J. Skaletsky Primer3 on the WWW for general users and for biologist programmers.

Lander but ongoing development and maintenance are not currently funded. Primer3 was a complete re-implementation of an earlier program: Primer 0. Primer3web version 3. Pick left primer, or use left primer below. Pick hybridization probe internal oligo , or use oligo below. Pick right primer, or use right primer below 5' to 3' on opposite strand.

Or mark the source sequence with [ and ]: e. Overlap Junction List. Or mark the source sequence with -: e. Excluded Regions. Pair OK Region List. Included Region. Start Codon Position. Internal Oligo Excluded Region. Force Left Primer Start. Force Right Primer Start. Force Left Primer End. Force Right Primer End. Min Sequence Quality. Min End Sequence Quality. Sequence Quality Range Min. Sequence Quality Range Max. Max Tm Difference. Table of thermodynamic parameters Breslauer et al.

Product Tm. Product Size Ranges. Number To Return. Max 3' Stability. Max Library Mispriming. Pair Max Library Mispriming. Max Template Mispriming.

Pair Max Template Mispriming. Max Tm Difference:. Table of thermodynamic parameters: Breslauer et al. Max Self Complementarity:. Max 3' Self Complementarity:. Max N's:. Max Poly-X:. Inside Target Penalty:. Outside Target Penalty:. Note: you can set Inside Target Penalty to allow primers inside a target. First Base Index:. Concentration of monovalent cations:. Salt correction formula:. Schildkraut and Lifson SantaLucia Owczarzy et. Concentration of divalent cations.

Concentration of dNTPs. Annealing Oligo Concentration:. Not the concentration of oligos in the reaction mix but of those annealing to template. Liberal Base. Show Debuging Info. Do not treat ambiguity codes in libraries as consensus.